Analysis of Gln aminoacyl synthetase.

Analysis performed on 2024-09-30 10:30:02

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 20031 265 19766 11532
Archaea 1501 31 1470 556
Vertebrata 761 243 518 352
Mammals 295 42 253 128

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
775 26 801

Domains

Domains
Current Study 1, 166
Guo et al (2010) 1, 260

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 610 165 775 3.696970
Number of Variants 639 188 827 3.398936
Number of Pathogenic Variants 20 6 26 3.333333
Number of Benign/Unknown Variants 619 182 801 3.401099
Fisher exact test 1.000000

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 515 260 775 1.980769
Number of Variants 535 292 827 1.832192
Number of Pathogenic Variants 20 6 26 3.333333
Number of Benign/Unknown Variants 515 286 801 1.800699
Fisher exact test 0.215644

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 610 165 0.90 (0.01) 0.81 (0.01) 9.753e-01 8.895e-01 71845.00 1.56e-17
Guo et al (2010) 515 260 0.91 (0.01) 0.82 (0.01) 9.840e-01 9.179e-01 95734.50 6.21e-23

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains